An audit was performed at The National Virus Reference Laboratory (NVRL) on the serological investigation of suspected Borrelia burgdorferi (B. burgdorferi) infection. The audit reviewed the performance of the B. burgdorferi IgM assays in comparison with the B. burgdorferi IgG and combined IgG/IgM assays. All samples in which there was serological evidence for recent B. burgdorferi infections were sent to the Lyme Borreliosis Unit, Southampton, UK for confirmation.